Expansion of human autologous cytotoxic T lymphocytes on fixed target tumor cells.
Authors:Horiuchi K1, Tsurushima H, Soo Kim B, Qin Liu S, Saijo K, Saijo Y, Nukiwa T, Nomura N, Matsumura M, Ohno T.
Abstract:Human tumor specific cytotoxic T lymphocytes (CTL) were expanded on formalin-fixed autologous target tumor cells derived from glioblastoma multiforme. Growth response of the CTL restimulated with the fixed target cells was larger than those with live target cells. The results suggest that formalin-fixed tumor cells will be stable sources of tumor antigen for efficient autologous CTL expansion and be useful for adoptive immunotherapy of tumors.